SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-22 12:18:20

description

N-acetylmuramoyl-L-alanine amidase

N-acetylmuramoyl-L-alanine amidase, required for the formation of membrane vesicles in a sub-population of cells

locus

BSU12810

BSU_12810

geneLength

891

894

outlinks

bsu

BSU12810

BSU_12810

Gene

Coordinates

1,347,289 → 1,348,182

1,347,289 1,348,182

The protein

Catalyzed reaction/ biological activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

The protein

Paralogous protein(s)

[[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]]

[[this]]

The protein

[SW|Domains]

contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]

contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])

contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]

contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|CwlH]], [[protein|XlyB]])

[SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 45-140) (according to UniProt)

[SW|LysM domain] (aa 159-203) (according to UniProt)

References

Original publications

9555893, 21816821, 7921239, 18957862

9555893, 21816821, 7921239, 18957862, 28883390

The protein

Protein family

[SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

Biological materials

Mutant

BKE12810 ([[gene|xlyA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTCTCCTTATT, downstream forward: _UP4_TGATCAAAGACCATAAAAAT

BKK12810 ([[gene|xlyA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCTCTCCTTATT, downstream forward: _UP4_TGATCAAAGACCATAAAAAT